The Candystripe’s Ryan McBride Brandywell ground fails to meet the required criteria to host the match.
Derry City designated the Tallaght Stadium in Dublin as an alternative.
However, Derry asked Uefa if they could switch the game to Windsor Park.
Uefa said the game must be played in “the same or another city within the territory of its association’.
Tallaght Stadium is now confirmed as the venue for the second leg of the third round qualifier against Kazakhstan’s Tobol on Thursday, August 17.
Derry wanted the game moved to Windsor Park in Belfast where Linfield play their home games, as it has a larger capacity than Tallaght and to reduce travel time for their fans.
The Candystripes were members of the Irish League until 1972 when clubs voted not to play at the Brandywell due to security concerns during The Troubles. They then joined the League of Ireland in 1985.
Uefa regulations stipulate that the venue announced at the beginning of the competition is considered as the ‘home venue’ of the respective club.
“A venue may be the ground of the home club or another ground in the same or another city within the territory of its association,” said a Uefa spokesperson.
“Therefore, Derry City FC will have to play in Tallaght as originally announced for the UECL 3rd qualifying round home fixture on the 17 August 2023.”
Uefa will only make exceptions should no other suitable venue be available on the ‘home territory’.
Derry will take on Tobol Kostanay in the first leg of the third round qualifier in Kazakhstan today, Thursday, August 10 (kick off 3 pm).